All links open original lab publication PDF files in a new tab. For more info on the book ‘The Self-Assembling Brain’ visit selfassemblingbrain.com.

2024

Agi, E.*, Reifenstein, E.T.*, Wit, C., Schneider, T., Kauer, M., Kehribar, M., Kulkarni, A., von Kleist, M.*, and Hiesinger, P.R.* (2024). Axonal Self-Sorting Without Target Guidance in Drosophila Visual Map Formation. Science, 2024 Mar 8;383(6687):1084-1092.

Wolterhoff, N. and Hiesinger, P.R. (2024). Synaptic Promiscuity in Brain Development. Curr Biol. 2024 Feb 5;34(3):R102-R116.

2023

Fuchs, J. and Hiesinger, P.R. (2023). Brain wiring: Love the one you’re with. Curr Biol. (Dispatch). 33(13):R727-R729

Daumann, I.-M. and Hiesinger, P.R. (2023). Lipid rafts, rab GTPases and a late endosomal checkpoint for plasma membrane recycling. Proc. Natl. Acad. Sci. USA, 120(14):e2302320120.

Hassan B.A. and Hiesinger, P.R. (2023). Autophagy in Synapse Formation and Brain Wiring. Autophagy. 19(10):2814-2816.

Nabavi, M. and Hiesinger, P.R. (2023). Turnover of Synaptic Adhesion Molecules. Mol. Cell. Neurosci. 124:103816.

Dutta, S.B., Linneweber, G.A., Andriatsilavo, M., Hiesinger, P.R.*, and Hassan, B.A.* (2023). EGFR-dependent suppression of synaptic autophagy is required for neuronal circuit development. Curr. Biol., 33, 1-16. * co-corresponding authors.

2022

Wit, C. and Hiesinger, P.R. (2022). Neuronal Filopodia: From Stochastic Dynamics to Robustness of Brain Morphogenesis. Sem. Cell Dev. Biol. https://doi.org/10.1016/j.semcdb.2022.03.038.

2021

Kiral, F.R., Dutta, S.B., Linneweber, G.A., Poppa, C., Duch, C., von KLeist, M., Hassan, B.A., and Hiesinger, P.R. (2021). Brain Connectivity inversely scales with developmental temperature in Drosophila, Cell Rep. 37(12):110145.

Kohrs, F.E*, Daumann, I.M.*, Pavlović, B., Jin, E.J., Lin, S.C. Port, F., Kiral, F.R., Wolfenberg, H., Mathejczyk, T.F., Linneweber, G.A., Chan, C.C., Boutros, M., Hiesinger, P.R. (2021) Systematic functional analysis of Rab GTPases reveals limits of neuronal robustness to environmental challenges in flies eLife 10:e59594 * co-first authors

Hiesinger, P.R. (2021). Brain Wiring with Composite Instructions. BioEssays e2000166.

2020

Kessissoglou I.A., Langui D., Hasan A., Maral M., Dutta S.B., Hiesinger P.R., Hassan B.A. (2020). The Drosophila amyloid precursor protein homologue mediates neuronal survival and neuroglial interactions. PloS Biology, 18(12):e3000703.

Kiral F.R., Linneweber G.A., Mathejczyk T.F., Georgiev S.V., Wernet M.F., Hassan B.A., von Kleist M., Hiesinger P.R. (2020). Autophagy-dependent filopodial kinetics restrict synaptic partner choice during Drosophila brain wiring, preprint at BioRxiv, Nature Communications, 12;11(1):1325.

Linneweber, G.A.,Andriatsilavo, M.,Dutta, S.,Bengochea, M.,Hellbruegge, L., Liu, G.,Ejsmont, R.K.,Straw, A.D., Wernet, M.F., Hiesinger, P.R., Hassan, B.A. (2020). A neurodevelopmental origin of behavioral individuality in the Drosophila visual system. Science, 367(6482):1112-1119.

Agi, E., Kulkarni, A., and Hiesinger, P.R. (2020). Neuronal strategies for meting the right partner during brain wiring. Curr. Opin. Neurobiol, 63:1-8.

2019

Ozel, M.N.*, Kulkarni, A.*, Hasan, A.*, Brummer, J., Moldenhauer, M., Daumann, I.M., Wolfenberg, H., Dercksen, V.J., Kiral, F.R., Weiser, M., Prohaska, S., von KLeinst, M.*, Hiesinger, P.R.* Serial synapse formation through filopodial competition for synaptic seeding factors, Developmental Cell 50(4):447-461.e8.

2018

Hiesinger, P.R. and Hassan, B.A. (2018). The Evolution of Variability and Robustness in Neural Development. Trends in Neuroscience 41(9):577-586

Jin E.J., Kiral, F.K., Özel, M.N., Burchardt, L.S., Osterland, M., Epstein, D., Wolfenberg, H., Prohaska, S., and Hiesinger, P.R. (2018). Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Curr. Biol Apr 2;28(7):1027-1038.e4

Kiral, F.K., Kohrs, F., Jin, E.J. and Hiesinger, P.R. (2018) Rab GTPases and Membrane Trafficking in Neurodegeneration. Curr Biol. Apr 23;28(8):R471-R486

2017

Jin E.J., Kiral, F.R. and Hiesinger, P.R. (2017) The Where, What and When of Membrane Protein Degradation in Neurons. Dev. Neurobiol. doi: 10.1002/dneu.22534

Özel, M.N. and Hiesinger, P.R. (2017) Live Imaging of Connectivity in Developing Neural Circuits in Drosophila. In: Decoding Neural Circuit Structure and Function, Springer 2017, p.149-167

Kolodkin, A.L. and Hiesinger, P.R. (2017) Wiring visual systems: common and divergent mechanisms and principles. Curr. Opin. Neurobiol. 42:128-135

2016

Klionsky et al., (2016)Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) Autophagy 12(1), 1–222

Garaulet, D.L., Sun, K., Li, W., Wen, J., Panzarino, A.M., O’Neil, J.L., Hiesinger, P.R., Young, M.W., and Lai, E.C. (2016) miR-124 Regulates Diverse Aspects of Rhythmic Behavior in Drosophila. J. Neurosci. 36(12):3414 –3421

Hiesinger, P.R. and Hassan, B.A. (2016) The Tightrope of Mentorship Cell Leading Edge: Voices, 164:1093

2015

Ozel, M.N., Langen, M., Hassan, B.A. and Hiesinger, P.R. (2015) Filopodial Dynamics and Growth Cone Stabilization in Drosophila Visual Circuit Development. eLife, e10721

Hassan, B.A. and Hiesinger, P.R. (2015) Beyond Molecular Codes: Simple Rules to Wire Complex Brain. Cell, 163(2):285-291 | [PDF]

Langen, M.*, Agi, E.*, Altschuler, D., Wu, L.*, Altschuler, S.*, and Hiesinger, P.R.* (2015) The Developmental Rules of Neural Superposition. Cell, 162(1):120-33 | [PDF] | [Suppl. Materials PDF]
Highlighted in: Wernet, M.F. and Desplan, C. (2015) Brain Wiring in the Fourth Dimension | Cell [PDF]

2014

Agi, E., Langen, M., Altschuler, S., Wu, L., Zimmermann, T., and Hiesinger, P.R. (2014) The Evolution and Development of Neural Superposition, J. Neurogenetics, 28(3-4):216-32. | [PDF]

Wang, D., Epstein, D., Khalaf, O., Srinivasan, S., Williamson, W.R., Fayyazuddin, A., Quiocho, F.A., and Hiesinger, P.R. (2014). Calcium/Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1. J. Cell. Biol. 205(1):21-31. | [PDF] | [Suppl. Mat. PDF]
  Highlighted in: Vavassori and Mayer (2014) J.Cell Biol. 205(1):7-99 | [PDF]

Zschatzsch, M., Oliva, C., Langen, M., De Geest, N., Ozel, M.N., Williamson , W.R., Lemon, W., Soldano, A., Munck, S., Hiesinger P.R., Sanchez-Soriano, N., Hassan, B. (2014) Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor Signaling. eLife, 3:e01699. | [PDF]

2013

Cherry, S., Jin, E.J., Ozel, M.N., Lu, Z., Agi, E., Wang, D., Meinertzhagen, I.A., Chan, C.-C., and Hiesinger, P.R. 2013. Charcot-Maire-Tooth 2B mutations in rab7 cause dosage-dependent neurodegeneration due to partial loss of function. eLife, 2(0):e01064 | [PDF]
  Highlighted in: Sadandappa, M.K. and Ramaswami, M. (2013) eLife e01820 | [PDF]

Wang, D. and Hiesinger, P.R. (2013). The vesicular ATPase: a missing link between acidification and exocytosis. J. Cell. Biol., 203(2):171-3 | [PDF]

Bezprozvanny, I., and Hiesinger, P.R. (2013). The synaptic maintenance problem: membrane recycling, Ca2+ homeostasis and late onset degeneration. Mol. Neurodegeneration, 8:23. | [PDF]

Carrerira-Rosario, A., Scoggin, S., Shalaby, N.A., Williams, N.D., Hiesinger, P.R. and Buszczak, M. (2013). Recombineering Homologous Recombination Constructs in Drosophila. J. Vis. Exp., in press. | [PDF] |

Wang, D., Chan, C.C., Cherry, S. and Hiesinger, P.R. (2013). Membrane trafficking in Neuronal Maintenance and Degeneration. Cell. Mol. Life Sci., 70(16):2919-34. | [PDF]

2012

Williamson, W.R., Chan, C.C., and Hiesinger, P.R. (2012). Subcellular Resolution Imaging in Neural Circuits. Bookchapter in: The Making and Un-Making of Neuronal Circuits, Bassem Hassan, Editor, Humana Press, p.61-90 | [PDF]

Jin, E.J., Chan, C.C., Agi, E., Cherry, S., Hanacik, E., Buszczak, M., and Hiesinger, P.R. (2012). Similarities of Drosophila rab GTPases Based on Expression Profiling: Completion and Analysis of the rab-Gal4 Kit. PLoS One, 7(7):e40912 | [PDF]

Coen, K. Flannagan, R.S., Carraro-Lacroix, L.R., Wang, D., Vermeire, W., Michiels, C. Munck, S., Sugita, S., Hiesinger, P.R. Grinstein, S., Annaert, W. (2012) Endo-lysosomal dysfunction in PSEN-deficient cells is caused by defects in lysosomal calcium homeostasis and not by defective proton pump function. J. Cell Biol., 198(1):23-35 | [PDF]

Wang, D., and Hiesinger, P.R. (2012). Autophagy, Neuron-Specific Degradation and Neurodegeneration. Autophagy, 8(4):711-3 | [PDF]

Haberman, A.S., Williamson, W.R., Epstein, D., Wang, D., Rina, S., Meinertzhagen, I.A. and Hiesinger, P.R. (2012). The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration in Drosophila. J. Cell. Biol., 196, 261-276. | [PDF] | [PDF] | [Cover]

Chan, C.C., Scoggin, S., Hiesinger, P.R.* and Buszczak, M.* (2012). Combining recombineering and ends-out homologous recombination to systematically characterize Drosophila gene families: rab GTPases as a case study. Comm. Integr. Biol., 5(2):179-83 * co-corresponding authors | [PDF]

2011

Chan, C.C., Scoggin, S., Wang, D., Cherry, S., Dembo, T., Greenberg, B., Jin, E.J., Kuey, C., Lopez, A., Mehta, S.Q., Perkins, T.J., Brankatschk, M., Rothenfluh, A., Buszczak, M.*, and Hiesinger, P.R.* (2011). Systematic Discovery of Rab GTPases with Synaptic Functions in Drosophila. Current Biology, 21(20):1704-15. * co-corresponding authors | [PDF] | [Supplemental Materials PDF]
  Highlighted in: Harris, K.P. and Littleton, J.T. (2011) Vesicle Trafficking: A Rab Family Profile. Curr. Biol. 21(20):841-843. | [Dispatch PDF]

Chan, C.C., Epstein, D., and Hiesinger, P.R. (2011). Intracellular Trafficking in Drosophila Visual System Development: A Basis for Pattern Formation through Simple Mechanisms. Dev. Neurobiol., 71(12):1227-45. | [PDF]

Smibert, P., Bejarano, F., Wang, D., Garaulet, D.L., Yang, J.-S., Martin, R., Bortolamiol-Becet, D., Robine, N., Hiesinger, P.R. and Lai, E.C.. (2011) A Drosophila genetic screen yields allelic series of core microRNA biogenesis factors and reveals post-developmental roles for microRNAs. RNA, 17(11):1997-2010 | [PDF]

2010

Williamson, W.R., Yang, T., Terman, J.R., and Hiesinger, P.R. (2010). Guidance Receptor Degradation is Required for Neuronal Connectivity in the Drosophila Nervous System. PLoS Biology, 8(12):e1000553 [PDF] | [Cover]

Williamson, W.R. and Hiesinger, P.R. (2010). On the role of v-ATPase V0a1-dependent degradation in Alzheimer Disease. Comm. Int. Biol. (in press) [PDF]

Williamson, W.R., Wang, D., Haberman, A.S. and Hiesinger, P.R. (2010). A dual function of V0-ATPase a1 provides an endolysosomal degradation mechanism in Drosophila photoreceptors. J. Cell. Biol., 189: 885-99. [PDF] | [Cover]
  Highlighted in: In Focus Article ‘The acid test of v-ATPase function’ by Ben Short, J. Cell. Biol. 189:773. [PDF]

Williamson, W.R. and Hiesinger, P.R. (2010). Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging. Journal of Visualized Experiments, 37. doi:10.3791/1936. [PDF] |

2009

Hiesinger, P.R. and Meinertzhagen, I.A. (2009) Visual System Development: Invertebrates. in: Squire LR (ed.) Encyclopedia of Neuroscience, volume 10, pp. 313-322. Oxford: Academic Press, Elsevier. [PDF]

2008

Williamson, W.R. and Hiesinger, P.R. (2008). Synaptic Patterning by Morphogen Signaling. Science Signaling, 1(18):pe20. [PDF]

Zhai, R.G., Zhang, F., Hiesinger, P.R., Cao, Y., Haeuter, C.M., and Bellen, H.J. (2008). NAD Synthase NMNAT acts as a chaperone to protect against neurodegeneration. Nature, 452:887-91. [PDF]

Zhang, W., Wang, D., Volk, E., Bellen, H.J., Hiesinger, P.R.*, Quiocho, F.A.* (2008) * co-corresponding authors
v-ATPase V0 sector subunit a1 in neurons is a target of calmodulin. Journal of Biological Chemistry, in press. [ PDF ]

Fischbach, K.F. and Hiesinger, P.R. (2007) Optic Lobe Development, in Brain Development of Drosophia, Ed. G. Technau, Landes Biosciences.   [ Pre-print PDF ] | [ Publisher link ]

2007

Zhang, J., Schulze, K.L., Hiesinger, P.R., Suyama, K., Wang, S., Fish, M., Acar, M., Hoskins, R.A., Bellen, H.J., and Scott, M.P. (2007) 31 Flavors of Drosophila Rab Proteins. Genetics 176:1307-1322.   [ PDF ] | [ PDF suppl ]

2006

Zhai, R.G., Cao, Y., Hiesinger, P.R., Mehta, S.Q., Schulze, K.L., Verstreken, P., Zhou, Y. and Bellen, H.J. (2006). Drosophila NMNAT Maintains Neural Integrity Independent of its NAD Synthesis Activity. PloS Biology 4(12):e416. [PDF]

Hiesinger, P.R.* #, Zhai, R.G.*, Zhou, Y., Koh, T.-W., Mehta, S.Q., Verstreken, P., Schulze, K.L., Cao, Y., Fischbach, K.-F., Meinertzhagen, I.A., and Bellen, H.J.#, (2006). Activity-independent prespecification of synaptic partners in the visual map of Drosophila Curr. Biol., 16, 1835-43 *co-first authors, # corresponding authors [PDF] | [PDF suppl]

Fayyazuddin, A., Zaheer, M., Hiesinger, P.R., and Bellen, H.J. (2006) giant fiber A (gfA) encodes a nicotinic acetylcholine receptor that mediates synaptic transmission in the giant fiber circuit of Drosophila.PloS Biology 4(3):e63. [PDF]

2005

Hiesinger, P.R. and Hassan, B.A. (2005). Genetics in the Age of Systems Biology. Cell, 123, 1173-4. [PDF]

Hiesinger, P.R., Fayyazuddin, A., Mehta, S.Q., Rosenmund, T., Schulze, K.L., Zhai, R.G., Verstreken, P., Cao, Y., Zhou, Y., Kunz, J., and Bellen, H.J. ( 2005). The v-ATPase V0 subunit is required for a late step in synaptic vesicle exocytosis in Drosophila. Cell 121, 607-620. Cover article. [PDF] | [PDF suppl] | [Cover]
 Preview in Cell 121, 496-497. Bajjalieh, S.A new view of an old pore.

Mehta, S.Q.*, Hiesinger, P.R.*, Beronja, S., Zhai, R.G., Schulze, K.L., Verstreken, P., Cao, Y., Zhou, Y. Tepass, U., Crair, M.C., and Bellen, H.J. (2005). *co-first authors Mutations in Drosophila sec15 reveal a function in neuronal targeting for a subset of exocyst components. Neuron 46, 219-232. [PDF]
 Preview in Neuron 46, 164-166. Clandinin, T.R. Surprising twists to exocyst function.

2004

Meinertzhagen I.A. and Hiesinger, P.R. (2004). Visual system development, invertebrates. Elsevier’s Encyclopedia of Neuroscience [link]

Hiesinger, P.R. and Bellen H.J.(2004). Flying in the face of total disruption. Nature Genetics 36, 211-212. [PDF]

Bellen, H.J., Levis, R.W., Liao, G., He, Y., Carlson, J.W., Tsang, G., Evans-Holm, M., Hiesinger, P.R., Schulze, K.L., Rubin, G.M., Hoskins, R.A., and Spradling, A.C. (2004). The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. Genetics 167, 761-781. [PDF]

2003

Zhai, R.G.*, Hiesinger, P.R.*, Koh, T.-W., Verstreken, P., Schulze, K.L., Cao, Y., Jafar-Nejad, H., Norga, K.K., Pan, H., Bayat, V., Greenbaum, M.P., and Bellen, H.J. (2003). Mapping Drosophila mutations with molecularly defined P element insertions. Proc. Natl. Acad. Sci. USA 100, 10860-10865. *co-first authors [PDF]
Featured Highlight in Nature Reviews Genetics 4, 849. Casci, T. I can name it in three…

Verstreken, P., Koh, T.-W., Schulze, K.L., Zhai, R.G., Hiesinger, P.R., Zhou, Y., Mehta, S.Q., Cao, Y., Roos, J., and Bellen, H.J. (2003). Synaptojanin is recruited by Endophilin to promote synaptic vesicle uncoating. Neuron 40, 733-748. [PDF]
  Preview in Neuron 40, 665-667. Song, W. and Zinsmaier, K.E. Endophilin and Synaptojanin hook up to promote synaptic vesicle endocytosis.

Fabian-Fine, R., Verstreken, P., Hiesinger, P.R., Horne, J.A., Kostyleva, R., Zhou, Y., Bellen, H.J., and Meinertzhagen, I.A. (2003). Endophilin promotes a late step in endocytosis at glial invaginations in Drosophila photoreceptor terminals. J. Neurosci. 23, 10732-10744. Cover article. [PDF] | [Cover]

2002

Morales, J., Hiesinger, P.R., Schroeder, A.J., Kume, K., Verstreken, P., Jackson, F.R., Nelson, D.L., and Hassan, B.A. (2002). Drosophila Fragile X protein, DFXR, regulates neuronal morphology and function in the brain. Neuron 34, 961-972. [PDF]

Pennetta, G., Hiesinger, P.R., Fabian-Fine, R., Meinertzhagen, I.A., and Bellen H.J. (2002). Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner. Neuron 35, 291-306. Cover article. [PDF] | [Cover]

Kango-Singh, M., Nolo, R., Tao, C., Verstreken, P., Hiesinger, P.R., Bellen, H.J., and Halder, G. (2002). Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila. Development 129, 5719-5730. Cover article. [PDF] | [Cover]

2001

Hiesinger, P.R.* , Scholz, M.*, Meinertzhagen, I.A., Fischbach, K.-F., and Obermayer, K. (2001). Visualization of synaptic markers in the optic lobe neuropils of Drosophila using a new constrained deconvolution method. J. Comp. Neurol. 429, 277-288. *co-first and corresponding authors. [PDF]

2000

Hiesinger, P.R. (2000). The evolution of the millenium bug. Riv. Biol./Biology Forum 93, 169-174.

1999

Hiesinger, P.R., Reiter, C., Schau, H., and Fischbach, K.-F. (1999). Neuropil pattern formation and regulation of cell adhesion molecules in Drosophila optic lobe development depend on synaptobrevin. J. Neurosci.19, 7548-7556. Cover article. [PDF]

Laissue, P.P., Reiter, C., Hiesinger, P.R., Halter, S., Fischbach, K.-F., and Stocker, R.F. (1999). Three-dimensional reconstruction of the antennal lobe in Drosophila melanogaster. J. Comp. Neurol.405, 543-552. [PDF]